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Engineering - Biosystems

Validation of Gold Nanoparticle DNA-based Biosensors for the Detection of Non-PCR Amplified Bacterial Food Borne Pathogens in Food Matrices

Principal Investigator: 
Sylvia Vetrone
Since the intentional distribution of Bacillus anthracis spores through the US postal system in 2001, the prospect of bioterrorism in food, water, and agriculture has identified critical needs in prevention, protection, and mitigation for homeland security. Towards that end, we recently...

Development of a Multiplex Bio-barcode DNA Biosensor for Bacillus anthracis Phase II

Principal Investigator: 
Evangelyn Alocilja
The long-term goal of the above project is to develop a DNA-based detection device that will not require PCR amplification and still retain PCRsensitivity. For this particular project, the deliverables include: (1) Synthesis and characterization of the DNA probe-coated and bio-barcoded...

Validation of Agent Inactivation and Isolation from Food Systems

Principal Investigator: 
Alvin Lee
Validation of a process needed to eliminate a microbiological or chemical hazard in food can be difficult. Processing technologies that have shown efficacy in eliminating a hazard under laboratory conditions, usually under well controlled parameters, may not be effective against the threat agent...

Validation and optimization of biologically modified electrically-active magnetic nanoparticles (nano-BEAMs) for direct capture and concentration of bioterrorism agents in various food matrices

Principal Investigator: 
Evangelyn Alocilja
The existing project focuses on the synthesis and novel application of biologically modified electrically-active magnetic nanoparticles (nano-BEAMs) for sample acquisition and pre-analytical processing to render representative samples of food products ready for rapid agent detection in multiple...

Proof-of-concept study for the development of a cell-free GPCR-based biosensor for rapid non-specific detection of chemical and biological toxic agents

Principal Investigator: 
Evangelyn Alocilja
This project is a high-risk/high-reward exploratory proof-of-concept work to evaluate the potential of G-protein coupled receptors (GPCRs) as sensing elements in biosensor devices for rapid non-specific but highly sensitive detection (detect-to-warn) of toxicants in selected complex food matrices...

Development of a Multiplex Bio-barcode DNA Biosensor for Bacillus anthracis Detection without PCR Amplification Phase I

Principal Investigator: 
Evangelyn Alocilja
The multiplex DNA-based detection device proposed here is novel and unique: the first design of a bio-barcode DNA amplification for Bacillus anthracis that uses a direct charge transfer (DCT) biosensor for signal measurement. The novelty is in the PCR-less DNA recognition coupled with rapid...

Development of Biologically Modified Electrically-active Magnetic Nanoparticles (nano-BEAMS) for Direct Capture and Concentration of Bacillus anthracis Spores and Cells in Various Food Matrices

Principal Investigator: 
Evangelyn Alocilja
This project has 4 objectives, namely: (1) to synthesize and characterize electrically-active magnetic nanoparticles, (2) to biologically modify the nanoparticles (nano-BEAMs) with antibodies and aptamers specific to the target Bacillus anthracis and optimize the binding parameters, (3) to evaluate...
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