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Molecular/cellular biology, genetics

Development of a Multiplex Bio-barcode DNA Biosensor for Bacillus anthracis Phase II

Principal Investigator: 
Evangelyn Alocilja
The long-term goal of the above project is to develop a DNA-based detection device that will not require PCR amplification and still retain PCRsensitivity. For this particular project, the deliverables include: (1) Synthesis and characterization of the DNA probe-coated and bio-barcoded...

Protein Complementation Based Functional Detection Assay for Staphylococcal Enterotoxin B

Principal Investigator: 
Jody Proescher
Staphylococcal enterotoxin B (SEB) is a significant cause of foodborne enteric illness and a potential biowarfare agent. A need exists for more rapid and sensitive methods to test food samples for SEB contamination with minimal sample manipulation and without complex instrumentation. A novel non-...

Chemical Inactivation of Protein Toxins on Food-Contact Surfaces

Principal Investigator: 
Jack Cappozzo
Ricin and abrin are potent plant toxins found in seeds of the castor bean plant and rosary pea plant, respectively. Both toxins have potential for being used as biological weapons since they are relatively easy to isolate and purify and can be disseminated as food contaminants. In the case of a...

Impact of Select Rodenticides on the Coagulation Properties of Milk Proteins

Principal Investigator: 
Salam Ibrahim
During enzymatic coagulation of milk, three separate but overlapping processes occur: (1) enzymatic hydrolysis, (2) aggregation, and (3) gelation. These processes must occur successfully in order to produce a desirable milk coagulum for cheese production. Key to the success of these processes is...

Inactivation of Microbial Agents with High Concentrations of Industrial Sanitizers

Principal Investigator: 
Katherine Swanson
This study evaluated the effectiveness of currently available chemical biocides against Bacillus anthracis spores and Yersinia pseudotuberculosis (as a surrogate for Y. pestis) on clean and soiled stainless steel surfaces. Information on the effect of available biocides on the above organisms is...

FASTMAN Integrated Device for Detection of Select Agents

Principal Investigator: 
Vivek Kapur
Our long-term goal is to develop assays for the sensitive, specific, and reliable detection of select agents in the food supply at the field, processing plant, distribution system, or at point-of-sale/use. Our immediate objectives have been to develop and validate accurate and convenient analytic...

A Systematic Approach for the Detection of Bioterrorism Agents in Complex Sample Matrices

Principal Investigator: 
Lee Ann Jaykus
The purpose of this project was to develop selective ligands (peptide and nucleic acid) to facilitate pre-analytical capture and subsequent detection of priority pathogens and bioagents (ricin B chain and B. anthracis spores) in foods. Twenty peptides displaying affinity to ricin B chain have been...

A Simple and Rapid Method for the Simultaneous Purification and Concentration of Nucleic Acids and Proteins from Food Matrices

Principal Investigator: 
Charles Young
The objective of this research is to evaluate the potential for an electrophoretic method known as isotachophoresis to simultaneously concentrate and purify nucleic acids and proteins from difficult food matrices. The method, initially developed using Internal Research and Development (IRAD) money...

Extraction of Bacillus anthracis Spores and Ricin from Liquid Foods and its Potential Utilization for Inline Detection

Principal Investigator: 
Francisco Diez-Gonzalez
The major objectives of this project are to identify a method for efficient extraction and concentration of Bacillus anthracis spores and ricin toxin from milk and orange juice, and to identify compatible rapid detection technologies. In addition to the major project objectives, a method for PCR...

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